Banco Nacional de Lineas Celurares


The National Stem Cell Bank of Stem Cell Lines (Banco Nacional de Líneas Celulares, BNLC) is a network structure under the umbrella of the Instituto de Salud Carlos III (ISCIII), based in Madrid. Its goal and legal mandate is to guarantee the preservation and hence the availability and distribution of pluripotent stem cell lines including human embryonic (hESC) and human induced pluripotent (iPS) stem cell lines produced in Spanish laboratories for use in biomedical research. There is a legal obligation to register, deposit and bank all the pluripotent stem cell lines generated in Spain. Availability cell line request at this web page.

The BNLC's functions also include the promotion of the quality and safety of its technical procedures. Data confidentiality, principles of precautionarity, proportionality and absence of profit must also be maintained in accordance with current Spanish regulations.

The BNLC role also includes the promotion of the the quality and safety of its technical procedures. Data confidentiality, precautionary, proportionality and absence of profit principles must also be maintained in accordance with current Spanish regulations

The BNLC also supports the researchers with the following additional services:

  • Management of Pluripotent Stem Cell projects. Advice and management on ethical, legal and administrative issues of research projects focused on pluripotent cells. Project management.
  • Pluripotent Stem Cell line generation:
    • iPS line generation and characterization: from biopsy or frozen cells.
    • hESC line derivation and characterization
  • Pluripotent Stem Cell line characterization:
    • Pluripotency markers.
    • In vitro differentiation.
    • In vivo differentiation.
    • Kariotyping and genotyping.
    • Microsatellite genotyping.

The BNLC is composed by four nodes located in Barcelona, San Sebastián, Valencia and the coordinating node in Granada.

Online documents: